Victor Cunha*, Clarissa Souza**, Elena Contreras§, Tiago Nunes*eRaphael Rocha*
A/V Clínica Veterinária, Rio de Janeiro, RJ, Brasil; +FDA Allergenic, Rio de Janeiro, RJ, Brasil; **Department of Veterinary Clinical Medicine, University of Illinois, Urbana, IL, USA; §Department of Clinical Sciences, Colorado State University, Fort Collins, CO, USA
Prick (percutaneous) tests have been used in human medicine for decades for the evaluation of skin sensitization towards environmental allergens1,2. The objective of this study was to evaluate the ability of the prick test to identify sensitization to house dust mites in dogs with clinically diagnosed atopic dermatitis. Ten healthy dogs and 45 dogs with atopic dermatitis were enrolled. Histamine and saline solutions were used as positive and negative controls, respectively. Three solutions ofDermatophagoides farinae (DF), D. pteronyssinus (DP) and Blomia tropicalis (BT) (FDA Allergenic, Rio de Janeiro, RJ, Brazil) were tested. Prick tests were performed in duplicate according to Duotip-II manufacturer recommendations (Lincoln Diagnostics Inc, Decatur, IL 62526). A positive response to an antigen was defined as wheals having a mean diameter of at least 3mm larger than the mean diameter of negative control wheals. One of the 10 healthy dogs had a positive response on the prick test whereas 30 of the 45 dogs with atopic dermatitis had a positive response. The odds of having a positive antigen response on the prick test were greater among dogs with atopic dermatitis (OR 18.0; 95% CI, 2.1 to 155.6; P=0.002). Of the 30 positive dogs with atopic dermatitis, three had positive responses to one allergen (1 DF, 2 DP). Eighteen to twodifferent mites allergens (10 DF and DP, 6 DF and BT, 2 DP and BT). Nine of the 30 dogs had positive responses to all three mites allergens (Figure 1). Prick Test is a potentially safe tool to evaluate house dust mite sensitization in dogs with atopic dermatitis.
APRESENTADO DURANTE O IX ONLINE CONGRESSO MUNDIAL DE DERMATOLOGIA VETERINÁRIA, 2020.