J. Possebom* 1 , V. Cunha2 , M. Lima3 , M. Farias4
In warm climates, allergens from house dust mites, such as: Dermatophagoides pteronyssinus (DP), Dermatophagoides farinae (DF) and Blomia tropicalis (BT) are a common cause of sensitization in dogs with atopic dermatitis (AD). The aim of this study was to identify specific IgE to allergens of house dust mites in the serum of dogs with AD sensu stricto. Twenty-one atopic dogs, dignosed as described by Favrot et al, after exclusively feeding a novel protein diet for eight weeks, were enrolled in the study. All dogs were positive in IDT to at least one of the mites tested. It was performed a 15% polyacrylamide gel SDS-PAGE and monoclonal anti-IgE Western blotting. Among all dogs, 13 (59%) reacted to BT and DP, and 10 (45.5%) to DF. The dogs sensitive to DP showed response against allergens in the range of 21.5-31kDa, suggesting response to Der p1 (25kDa), Der p3 (31kDa), Der p6 (25kDa) and Der p9 (29kDa). Seven dogs (53.8%) showed response in the range of 14.5-21.4kDa, probably Der p2 (15kDa). Among the 10 dogs sensitive to DF, 100% showed response between 21.6-31kDa, probably Der f1 (25kDa); while 6/10 (60%) responded to allergens with more than 97.4kDa, suggesting response to Der f15 (98kDa); and 5/10 (50%) showed response in the range of 66.2-97.4kDa. Among the 13 dogs sensitive to BT, six (46.1%) reacted in the range of 21.5- 31kDa, indicating response to Blo t6 (25kDa); and 5/13 dogs (38.5%) reacted in the range of 66.2-97.4kDa. In conclusion, dogs with AD may have serum IgE against low molecular weight allergens, which have enzymatic nature, are commonly found in house dust, and have been responsible for the development of allergic diseases in humans.
2016