J. Possebom* 1 , M. Lima2 , V. Cunha3 , M. Farias4
House dust mite allergens of high molecular weight are usually implicated in canine atopic dermatitis (AD). This study aimed to identify molecular weight of the proteins to which IgE from atopic dogs reacted against. Twenty client-owned atopic dogs were enrolled in the study after confirming that they fulfilled six of eight clinical features of canine AD, as described by Favrot et al, ruling out other pruritic dermatoses, and exclusively feeding a novel protein diet for eight weeks. Intradermal and FceR1-α serological tests were performed in all 20 atopic dogs to identify sensitization to D. pteronissynus (DP). SDS-PAGE with a 15% gel and a monoclonal anti-dog IgE western blotting were used to identify molecular weight of reacting allergens. Among the 20 dogs tested, 13 (65%) showed positive results for DP in intradermal, serological and western blotting tests. All 13 dogs reacted against proteins in the range of 21,5-31kDa, what may indicate reaction to Der p1 (25kDa), Der p3 (31kDa), Der p6 (25kDa) and Der p9 (29kDa). Seven of the 13 dogs (53,8%) reacted against proteins in the range of 14,5-21,5kDa, what may indicate response against Der p2 (15kDa); while 4/13 dogs (30,8%) showed reactions between 31,1-44,9kDa, possibly to Der p10 (36kDa); and 1/13 (7,7%) dogs showed reactions to proteins of less than 14,4kDa, such as Der p5 (14kDa). Low molecular weight DP allergens, can cause sensitization and eczema precipitation in dogs with atopic dermatites usually implicated in humans with allergic rhinitis and asthma.
2016