SEROLOGICAL IDENTIFICATION OF LOW WEIGHT ALLERGENS FROM DOMESTIC MITES ASSOCIATED WITH CANINE ATOPIC DERMATITIS

 J. Possebom* 1 , V. Cunha2 , M. Lima3 , M. Farias4

In warm climates, allergens from house dust mites, such as: Dermatophagoides pteronyssinus (DP), Dermatophagoides farinae (DF) and Blomia tropicalis (BT) are a common cause of sensitization in dogs with atopic dermatitis (AD). The aim of this study was to identify specific IgE to allergens of house dust mites in the serum of dogs with AD sensu stricto. Twenty-one atopic dogs, dignosed as described by Favrot et al, after exclusively feeding a novel protein diet for eight weeks, were enrolled in the study. All dogs were positive in IDT to at least one of the mites tested. It was performed a 15% polyacrylamide gel SDS-PAGE and monoclonal anti-IgE Western blotting. Among all dogs, 13 (59%) reacted to BT and DP, and 10 (45.5%) to DF. The dogs sensitive to DP showed response against allergens in the range of 21.5-31kDa, suggesting response to Der p1 (25kDa), Der p3 (31kDa), Der p6 (25kDa) and Der p9 (29kDa). Seven dogs (53.8%) showed response in the range of 14.5-21.4kDa, probably Der p2 (15kDa). Among the 10 dogs sensitive to DF, 100% showed response between 21.6-31kDa, probably Der f1 (25kDa); while 6/10 (60%) responded to allergens with more than 97.4kDa, suggesting response to Der f15 (98kDa); and 5/10 (50%) showed response in the range of 66.2-97.4kDa. Among the 13 dogs sensitive to BT, six (46.1%) reacted in the range of 21.5- 31kDa, indicating response to Blo t6 (25kDa); and 5/13 dogs (38.5%) reacted in the range of 66.2-97.4kDa. In conclusion, dogs with AD may have serum IgE against low molecular weight allergens, which have enzymatic nature, are commonly found in house dust, and have been responsible for the development of allergic diseases in humans.

2016

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DIAGNOSTIC ACCURACY OF SERUM ASSAYS FOR MITE SENSITIVITY IN DOGS WITH ATOPIC DERMATITIS SENSU STRICTO

 M. LIMA* 1 , V. CUNHA2 , M. FARIAS3

This study aimed to evaluate accuracy parameters of a polyclonal and a FceR1-alfa serological test, compared to IDT, for identification of sensitivity to Dermatophagoides pteronyssinus (DP), Dermatophagoides farinae (DF) and Blomia tropicalis (BT). This study enrolled 19 healthy dogs with negative IDT and 20 atopic dogs with positive IDTand no other pruritic dermatoses, that fulfilled six of eight clinical features of canine AD, as described by Favrot et al, after exclusively feeding a novel protein diet for eight weeks. Serological testing was performed in all 39 dogs. When considered positive result to at least one of the tested mites (overall rating), polyclonal serological test showed accuracy=0,49, sensitivity (S)=74%, specificity (E)=26%. When considering results of each mite tested individually, this test showed for DP: accuracy=0,58, S=36%, E=74%; for DF: accuracy=0,46, S=78%, E= 32% and for BT:accuracy=0,53, S= 55%, E= 53%. FceR1-alpha test showed in overall rating: accuracy=0,46, S=65%, E=26%; in mite rating, for DP:accuracy=0,58, S=57%, E=58%; for DF: accuracy=0,50, S=44%, E=53% and for BT: accuracy=0,67, S=45%, E= 79%. 

2016

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IDENTIFICATION OF DERMATOPHAGOIDES PTERONYSSINUS ALLERGENS IN DOGS WITH ATOPIC DERMATITIS SENSU STRICTO POSITIVE IN INTRADERMIC AND SEROLOGICAL (FCER1-ALPHA) TESTING

  J. Possebom* 1 , M. Lima2 , V. Cunha3 , M. Farias4

House dust mite allergens of high molecular weight are usually implicated in canine atopic dermatitis (AD). This study aimed to identify molecular weight of the proteins to which IgE from atopic dogs reacted against. Twenty client-owned atopic dogs were enrolled in the study after confirming that they fulfilled six of eight clinical features of canine AD, as described by Favrot et al, ruling out other pruritic dermatoses, and exclusively feeding a novel protein diet for eight weeks. Intradermal and FceR1-α serological tests were performed in all 20 atopic dogs to identify sensitization to D. pteronissynus (DP). SDS-PAGE with a 15% gel and a monoclonal anti-dog IgE western blotting were used to identify molecular weight of reacting allergens. Among the 20 dogs tested, 13 (65%) showed positive results for DP in intradermal, serological and western blotting tests. All 13 dogs reacted against proteins in the range of 21,5-31kDa, what may indicate reaction to Der p1 (25kDa), Der p3 (31kDa), Der p6 (25kDa) and Der p9 (29kDa). Seven of the 13 dogs (53,8%) reacted against proteins in the range of 14,5-21,5kDa, what may indicate response against Der p2 (15kDa); while 4/13 dogs (30,8%) showed reactions between 31,1-44,9kDa, possibly to Der p10 (36kDa); and 1/13 (7,7%) dogs showed reactions to proteins of less than 14,4kDa, such as Der p5 (14kDa). Low molecular weight DP allergens, can cause sensitization and eczema precipitation in dogs with atopic dermatites usually implicated in humans with allergic rhinitis and asthma.

2016

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INTRADERMAL TESTS – PROFICIENCY TESTING IN DOGS

1 Victor E S Cunha, 2 Desydere T Pereira, 3 Marconi R Farias, 4 Rafael R Ferreira and 5 Alexandre Krause
1Department of Research and Development, FDA Allergenic, Brazil (victor@fda-­‐allergenic.com.br) 2Department of Small Animal Clinical Sciences, Universidade Federal de Santa Maria, Brazil. 3College of Agrarian Sciences and Veterinary Medicine, Pontifícia Universidade Católica do Paraná, Brazil. 4College of Veterinary, Universidade Federal do Rio Grande do Sul, Brazil.
Objective: of this study was to propose a proficiency test for IDT in dogs that would serve as a starting point for veterinarians who want to perform IDT for the specific diagnosis of CAD.

2015 | VER PDF

SENSIBILIZAÇÃO A ÁCAROS DOMICILIARES EM CÃES COM DERMATITE ATÓPICA E CONCORDÂNCIA ENTRE TESTE INTRADÉRMICO E TESTE SOROLÓGICO

Victor do Espirito Santo Cunha, Doutor – FDA Allergenic Ltda; João Luiz Horacio Faccini, Doutor – UFRRJ
OBJETIVO: avaliar a importância de anIgenos de ácaros domiciliares (AD) para a DAC na cidade do Rio de Janeiro e a concordância entre o Teste Intradérmico (TID) e Teste Sorológico (TSO)

2013 | VER PDF

ANÁLISE COMPARATIVA DAS PRESCRIÇÕES MÉDICAS DE IMUNOTERAPIA NOS ANOS 2009 E 2015. O BRASIL SEGUE AS BOAS PRÁTICAS?

Ledit R. F. Ardusso1, Victor E. S. Cunha2, Tielli Magnus2, Jorge L. Molinas3, Ruppert L. Hahnstadt2
1-Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Argentina. 2-FDA Allergenic Group, Rio de Janeiro, Brasil. 3-Universidad del Centro Educativo Latinoamericano, Rosario, Argentina. 
Objetivo: Analisar características das prescrições para imunoterapia específica com alérgenos (SIT) no Brasil e avaliar se cumprem as recomendações dos guias internacionais. 

2015 | VER PDF

FRECUENCIA DE RESULTADOS POSITIVOS A PÓLENES DE GRAMÍNEAS EM TESTS INTRADÉRMICOS REALIZADOS EN 308 PERROS CON DERMATITIS ATÓPICA EN RIO GRANDE DO SUL, SUR DE BRASIL

1 Rafael R. Ferreira, 2 Desydere T Pereira e 3 Victor E S Cunha
1 Médico Veterinário autónomo, Doutor, Profesor Dermatología Veterinária, Brazil. 2 Programa de Postgrado en Medicina Veterinaria, Universidade    Federal de Santa Maria, Brazil. 3 Departamento de investigación y desarrollo, FDA Allergenic, Brazil.
Objetivo: evaluar la frecuencia de positividad a los pólenes de gramíneas: Cynodon dactylon, Paspalum notatum y Lolium mul

2015 | VER PPT